Contact an Account ManagerAutomated solid-phase extraction and two-step derivatization for simultaneous analysis of basic illicit drugs in serum by GC/MS
Summary
Type: SPE
Application #: 304000
Detection:
Conditions: Automated SPE with Zymark RapidTrace® SPE robot
Column conditioning: 2.0 mL methanol (2.0 mL/min), then 2.0 mL 0.1 mol/L phosphate buffer, pH 6.0 (2.0 mL/min)
Sample aspiration: 3.0 mL sample (1.0 mL/min)
Washing: 1.0 mL water (2.0 mL/min), then 1.0 mL 0.1 mmol/L acetic acid (2.0 mL/min), then 2.0 mL methanol (2.0 mL/min)
Drying: nitrogen (1.0 min)
Elution: 1.5 mL dichloromethane – 2-propanol – 25 % aqueous ammonia (80:20:2, v/v/v) (2.0 mL/min)
Evaporation: to 0.1 mL with nitrogen
Authors: Weinmann, W. et al.
Source: Int J Legal Med (2000) 113, 229–235
Details
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